New Recombinant Antimicrobial Peptides Confer Resistance to Fungal Pathogens in Tobacco Plants.
Identifieur interne : 000098 ( Main/Exploration ); précédent : 000097; suivant : 000099New Recombinant Antimicrobial Peptides Confer Resistance to Fungal Pathogens in Tobacco Plants.
Auteurs : Mitra Khademi [Iran] ; Marzieh Varasteh-Shams [Iran] ; Farhad Nazarian-Firouzabadi [Iran] ; Ahmad Ismaili [Iran]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
Antimicrobial peptides have been long known to confer resistance to plant pathogens. In this study, new recombinant peptides constructed from a dermaseptin B1 (DrsB1) peptide fused to a chitin-binding domain (CBD) from Avr4 protein, were used for Agrobacterium tumefaciens-mediated transformation of tobacco plants. Polymerase chain reaction (PCR), semi-quantitative RT-PCR, and western blotting analysis demonstrated the incorporation and expression of transgenes in tobacco genome and transgenic plants, respectively. In vitro experiments with recombinant peptides extracted from transgenic plants demonstrated a significant (P<0.01) inhibitory effect on the growth and development of plant pathogens. The DrsB1-CBD recombinant peptide had the highest antifungal activity against fungal pathogens. The expression of the recombinant peptides greatly protected transgenic plants from Alternaria alternata, Alternaria solani, Fusarium oxysporum, and Fusarium solani fungi, in comparison to Pythium sp. and Pythium aphanidermatum. Expression of new recombinant peptides resulted in a delay in the colonization of fungi and appearance of fungal disease symptoms from 6 days to more than 7 weeks. Scanning electron microscopy images revealed that the structure of the fungal mycelia appeared segmented, cling together, and crushed following the antimicrobial activity of the recombinant peptides. Greenhouse bioassay analysis showed that transgenic plants were more resistant to Fusarium and Pythium infections as compared with the control plants. Due to the high antimicrobial activity of the recombinant peptides against plant pathogens and novelty of recombinant peptides, this report shows the feasibility of this approach to generate disease resistance transgenic plants.
DOI: 10.3389/fpls.2020.01236
PubMed: 32903611
PubMed Central: PMC7438598
Affiliations:
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Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad</wicri:regionArea><wicri:noRegion>Khorramabad</wicri:noRegion></affiliation></author><author><name sortKey="Nazarian Firouzabadi, Farhad" sort="Nazarian Firouzabadi, Farhad" uniqKey="Nazarian Firouzabadi F" first="Farhad" last="Nazarian-Firouzabadi">Farhad Nazarian-Firouzabadi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad</wicri:regionArea><wicri:noRegion>Khorramabad</wicri:noRegion></affiliation></author><author><name sortKey="Ismaili, Ahmad" sort="Ismaili, Ahmad" uniqKey="Ismaili A" first="Ahmad" last="Ismaili">Ahmad Ismaili</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad</wicri:regionArea><wicri:noRegion>Khorramabad</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antimicrobial peptides have been long known to confer resistance to plant pathogens. In this study, new recombinant peptides constructed from a dermaseptin B1 (DrsB1) peptide fused to a chitin-binding domain (CBD) from Avr4 protein, were used for <i>Agrobacterium tumefaciens</i>-mediated transformation of tobacco plants. Polymerase chain reaction (PCR), semi-quantitative RT-PCR, and western blotting analysis demonstrated the incorporation and expression of transgenes in tobacco genome and transgenic plants, respectively. <i>In vitro</i> experiments with recombinant peptides extracted from transgenic plants demonstrated a significant (P<0.01) inhibitory effect on the growth and development of plant pathogens. The DrsB1-CBD recombinant peptide had the highest antifungal activity against fungal pathogens. The expression of the recombinant peptides greatly protected transgenic plants from <i>Alternaria alternata, Alternaria solani, Fusarium oxysporum</i>, and <i>Fusarium solani</i> fungi, in comparison to <i>Pythium</i> sp. and <i>Pythium aphanidermatum</i>. Expression of new recombinant peptides resulted in a delay in the colonization of fungi and appearance of fungal disease symptoms from 6 days to more than 7 weeks. Scanning electron microscopy images revealed that the structure of the fungal mycelia appeared segmented, cling together, and crushed following the antimicrobial activity of the recombinant peptides. Greenhouse bioassay analysis showed that transgenic plants were more resistant to <i>Fusarium</i> and <i>Pythium</i> infections as compared with the control plants. Due to the high antimicrobial activity of the recombinant peptides against plant pathogens and novelty of recombinant peptides, this report shows the feasibility of this approach to generate disease resistance transgenic plants.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32903611</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>New Recombinant Antimicrobial Peptides Confer Resistance to Fungal Pathogens in Tobacco Plants.</ArticleTitle><Pagination><MedlinePgn>1236</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.01236</ELocationID><Abstract><AbstractText>Antimicrobial peptides have been long known to confer resistance to plant pathogens. In this study, new recombinant peptides constructed from a dermaseptin B1 (DrsB1) peptide fused to a chitin-binding domain (CBD) from Avr4 protein, were used for <i>Agrobacterium tumefaciens</i>-mediated transformation of tobacco plants. Polymerase chain reaction (PCR), semi-quantitative RT-PCR, and western blotting analysis demonstrated the incorporation and expression of transgenes in tobacco genome and transgenic plants, respectively. <i>In vitro</i> experiments with recombinant peptides extracted from transgenic plants demonstrated a significant (P<0.01) inhibitory effect on the growth and development of plant pathogens. The DrsB1-CBD recombinant peptide had the highest antifungal activity against fungal pathogens. The expression of the recombinant peptides greatly protected transgenic plants from <i>Alternaria alternata, Alternaria solani, Fusarium oxysporum</i>, and <i>Fusarium solani</i> fungi, in comparison to <i>Pythium</i> sp. and <i>Pythium aphanidermatum</i>. Expression of new recombinant peptides resulted in a delay in the colonization of fungi and appearance of fungal disease symptoms from 6 days to more than 7 weeks. Scanning electron microscopy images revealed that the structure of the fungal mycelia appeared segmented, cling together, and crushed following the antimicrobial activity of the recombinant peptides. Greenhouse bioassay analysis showed that transgenic plants were more resistant to <i>Fusarium</i> and <i>Pythium</i> infections as compared with the control plants. Due to the high antimicrobial activity of the recombinant peptides against plant pathogens and novelty of recombinant peptides, this report shows the feasibility of this approach to generate disease resistance transgenic plants.</AbstractText><CopyrightInformation>Copyright © 2020 Khademi, Varasteh-Shams, Nazarian-Firouzabadi and Ismaili.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Khademi</LastName><ForeName>Mitra</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Varasteh-Shams</LastName><ForeName>Marzieh</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nazarian-Firouzabadi</LastName><ForeName>Farhad</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ismaili</LastName><ForeName>Ahmad</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList 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Marzieh" sort="Varasteh Shams, Marzieh" uniqKey="Varasteh Shams M" first="Marzieh" last="Varasteh-Shams">Marzieh Varasteh-Shams</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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